HRGFish

A resource on hypoxia responsive genes in fish

Primer design

Genomic mRNA primers of the gene camk2g (calcium/calmodulin dependent protein kinase II gamma) of species Astyanax mexicanus

Selected sub sequence

Target sequence length: 83507

Selected subsequence from: 1 to:700

Selected subsequence size:700

GTGTGCGCGAGACCAGCCTCCCCGAAACCTGAGCGAGGCAGAGGCAGCGAAACGAGAGGCGACAGAGCGCGAGCGCTACCACCACCCTCAGCACAACATGGCTACAATTGCAACCTCGAC
CAGGTTTACAGACGAGTACCAGCTCTACGAGGAGCTCGGAAAGTAAGCGACAGCTGCAGCTGCTGCTGCTGGTTTTGTGTTTGTTTGGTTGTTTATTTGGTCGGATAGTCCGCTCTCCGG
GCTGGTTCTGCGGGTCTGTGTGCTGAGCGGACTGCAGGAGCCGCTGGCTGTCTGTCGCCTGTCAGAAACAGCACGGCAACCAGGAAGCCTGTCTTCACCTTTTAGACCGATAGACAGGTT
AGAGGGCTGTGTGATCTCAGCCCCAGCGCTGCTAGGAGCTGTGTTCATGGTTTATGCTGTTGTTAAATTTGACTGCAAACAGTCGTTAAAACATCGTGTTAGCTCCGCTCCGGCGACCAG
AGCTCTTGAGCCTGCCTGTGTTTTTGTCGTGCCGTGTTCAATAAGGTGGCTGCTGCTCGCTCCAGCCTTCTGAGTGGCCGGTGTTCTGTACAGTCTTGCTACCCATCGATATCTGCTAGG
TGTCAGCACTGCGCATGCGCTAACCTAACATTTATTTTATGCTTTCGTGAGTTTTTTATTTTTAGTTTTGCTTGAGTGCGTTATACAATTTGAGCCTACT

Primers information

S.No.	Primers	Sequences	Start	Length	Tm	GC %	Product size
1	Forward primer Reverse primer	CAGAGGCAGCGAAACGAGAG AGACTGTACAGAACACCGGC	38 575	20 20	61.075 59.683	60.000 55.000	538 bp
2	Forward primer Reverse primer	GAAACGAGAGGCGACAGAGC AGACTGTACAGAACACCGGC	48 575	20 20	61.074 59.683	60.000 55.000	528 bp
3	Forward primer Reverse primer	AAACGAGAGGCGACAGAGC AGACTGTACAGAACACCGGC	49 575	19 20	60.080 59.683	57.895 55.000	527 bp
4	Forward primer Reverse primer	CGAAACGAGAGGCGACAGAG AGACTGTACAGAACACCGGC	47 575	20 20	60.795 59.683	60.000 55.000	529 bp
5	Forward primer Reverse primer	TACCACCACCCTCAGCACAA AGACTGTACAGAACACCGGC	76 575	20 20	61.057 59.683	55.000 55.000	500 bp

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