This similarity search program enables to users for performing alignment between their query sequence and FMiR database. There are choices for selection of dataset and program as per query requirement. Nucleotide datasets (Complete mt genome,Gene, D-loop and RNA) can be selected as target for DNA query sequence and alignment can be generated using Blastn program. Protein dataset can be used to generate sequence alignment for protein query using Blastp program and for DNA query using Blastx program.

Dataset:  Select mitochondrial dataset to be aligned with query    Complete mt genome Gene D-loop RNA Protein   Program:  Select dataset compatible Blast program for query    Blastp Blastn Blastx Parameters:   Word size        Maximum number of alignments        Minimum percent identity  Paste a mitochondrial DNA   protein sequence in FASTA format