HRGFish

A resource on hypoxia responsive genes in fish

Primer design

Genomic mRNA primers of the gene arnt2 (aryl-hydrocarbon receptor nuclear translocator 2) of species Danio rerio

Selected sub sequence

Target sequence length: 172496

Selected subsequence from: 1 to:700

Selected subsequence size:700

TTTAGTACACCAAAAAGAGATAAGCTAAGCTAATCCAGCTAAAACAGTGCTTAAAATTTCACCAAATTCAAACCTCATAATTAAATAAAAAAATGTAGTAAATTTGTTGTTATAGCCCCA
AAACTCTGGAACGGGCTTCCACCCCATATTAGACACGCTTTAACTCTATCTGTTTTTAAAAAGCTTCTCAAACAGCATCTTTGTTCTTTAGCATTTGAGCTTTTTTATGTTGAGGGGTTT
GTCTTCTATATTAATTCTTATGTATGTGATGTAGAACTTATTTTAATTAGTATTGATTGTGTTGTAAAAAAATGTGCTATATAAACTAACTAACGACTAATGTTGTCTTACATATTAGTG
ATTTTTCTAATATGCTTACAGTCAAATGCGAAAATTTTTAAATGACCAAATCTGCTTCTGGCAAAAGTCAGACTTCAAACCTGTCAGACAAAATTGAGGTTAAGTTGGTTTTATATACAC
ACATTTTCCTTAATAATATAATTTCTGAAGTATTAATTACAAGTTCCAAACACTAAACTTATTTGACTATAAAAGTACAACAACTTTCACAGTCAAATAAATAGCGCAAATGTTGTTTTG
AAGTAATATTTACATGTAATTTTAGACCGGATACTCAAAGTACCATGGTAAACAATATAGGAAGCATGTGTCACAGGTTGTCCCTGAACGCATGTGCTAA

Primers information

S.No.	Primers	Sequences	Start	Length	Tm	GC %	Product size
1	Forward primer Reverse primer	CCCCAAAACTCTGGAACGGG CACATGCGTTCAGGGACAAC	115 695	20 20	60.894 59.762	60.000 55.000	581 bp
2	Forward primer Reverse primer	CCAAAACTCTGGAACGGGCT GGACAACCTGTGACACATGC	117 682	20 20	60.537 59.407	55.000 55.000	566 bp
3	Forward primer Reverse primer	CCAAAACTCTGGAACGGGCT CACATGCGTTCAGGGACAAC	117 695	20 20	60.537 59.762	55.000 55.000	579 bp
4	Forward primer Reverse primer	AGCCCCAAAACTCTGGAACG GGACAACCTGTGACACATGC	113 682	20 20	60.537 59.407	55.000 55.000	570 bp
5	Forward primer Reverse primer	AGCCCCAAAACTCTGGAACG CACATGCGTTCAGGGACAAC	113 695	20 20	60.537 59.762	55.000 55.000	583 bp

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