HRGFish

A resource on hypoxia responsive genes in fish

Primer design

Genomic mRNA primers of the gene notch2 (notch receptor 2) of species Danio rerio

Selected sub sequence

Target sequence length: 70808

Selected subsequence from: 1 to:700

Selected subsequence size:700

CAGTGACTGGGACGAAAAGAAGAGAGAAGTTCAGAAAGTTTGATCACAGCGCAGCTACGTGAAGAAAAAAACTTTCAAATGAAGTCACTATACGGATTTATGTGGTCAAACGAGACACCC
TGACAGACAAAACACAGCTTCTCCACCGAGTATAATTCGTTTTCCGCGATTTGAAGAGCTTTTTGAGATTCGTGACGCTTTAACCTGACGGAGCTCAGCCTCGCGCTGGCGGCTCTATGT
GGATTTTACTCAAAATATGGGCCAATTACCCGGTGTCTCTTCGGGCGGAGTCATCCTTCTCCTTATTTGCTTTCTCAAAACATCATATGGTAAGTGGTTTAACAGTTTATATATCTGAAC
TCGTTCTTTTAAACCGCTATTTGCCGTCAGTAACGTTACGCATCTGTACGGCGGCGTGAAGCATCAGCGCTCACGTTTCAGTTCTGCACGGCCTCAAATGGCGTGTGTCCGCTCACGTTA
TTGGAAATTGACTAACATAATGAAGTAAAAGATGAGACATATTATTTACGCTTTTGCATAAACATTTAAAAACTCAAAGCTTTTTCCCCCCCGAAGTGAGCTCTCGTGTTTCACACCTCT
GCCCAGATGTGAAAGTCGGCGGATGAAATCTGCAGCGGCTTCCAGTAACTAATGTGAGAGGAGAATTCAAAATGGACACGTATGAGCGGAAAATATGGAC

Primers information

S.No.	Primers	Sequences	Start	Length	Tm	GC %	Product size
1	Forward primer Reverse primer	TGTGGTCAAACGAGACACCC TTCATCCGCCGACTTTCACA	100 626	20 20	60.179 59.966	55.000 50.000	527 bp
2	Forward primer Reverse primer	TGGTCAAACGAGACACCCTG TTCATCCGCCGACTTTCACA	102 626	20 20	59.894 59.966	55.000 50.000	525 bp
3	Forward primer Reverse primer	TGGTCAAACGAGACACCCTG TAGTTACTGGAAGCCGCTGC	102 650	20 20	59.894 60.108	55.000 55.000	549 bp
4	Forward primer Reverse primer	AACACAGCTTCTCCACCGAG TAGTTACTGGAAGCCGCTGC	130 650	20 20	59.966 60.108	55.000 55.000	521 bp
5	Forward primer Reverse primer	GTGGTCAAACGAGACACCCT TTCATCCGCCGACTTTCACA	101 626	20 20	59.894 59.966	55.000 50.000	526 bp

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