HRGFish

A resource on hypoxia responsive genes in fish

Primer design

Genomic mRNA primers of the gene flt1 (fms-related tyrosine kinase 1 (vascular endothelial growth factor/vascular permeability factor recep) of species Danio rerio

Selected sub sequence

Target sequence length: 58007

Selected subsequence from: 1 to:700

Selected subsequence size:700

AATTCATGCGCGGTCCTCCTGCTTCAAGAGTTCATACACATTGCGGAAACTAGCTACCGAGATTGACTGTTGTCCCCGTGAACGCAGGGTTTCAGGAGTTTGGCGTTGTGAGGTGAACTG
ATTCTCTGGAGTATTGGATCCGCAGTGTTATATTCCTCCATAGGTATTCCTTCATCTCCAAACAAACACCCTCAAGCAAGACCAAGAGAATGTTCGATATATTATTTGTGATGATATTTG
GACTGTCAGGACGTGTTCTGACTAAAGGTATGTGCTTTATTTTTAAGTGTTTTATTTGTGTTCGTATATAGCCTACAAATAATACATTTATGTATAGTCCATATATTAATTTTAGCTACT
TGTCATTGTATTCAAAATAAGTTCTTGTTTAACACTCATCAAAGTGATGAAATACAGGTCTGGAATGCGCGCGGATGAGAGTGTGGCATTGCTCAGCTCCAGCGCATCTGATACCTAGAA
AAAAACATCCAGAAACTTTGGATGCATATATAAACTTGTTCGCATATCTTTATTTTTAATAAACAATTCAATGGCAATTGAGAGTATGACATAATTGCAAAATTATGCAAGAAATTTGCG
CAAAAAAAATTATTTACTAAAATTTTAGTTTTCCCACTACTATGGCAAGCCGATTTAACATTTATCACAACTATGTACAAATACTAACTGGTTTGCGCTT

Primers information

S.No.	Primers	Sequences	Start	Length	Tm	GC %	Product size
1	Forward primer Reverse primer	TGACTGTTGTCCCCGTGAAC TCGGCTTGCCATAGTAGTGG	63 652	20 20	60.179 59.536	55.000 55.000	590 bp
2	Forward primer Reverse primer	AGTTTGGCGTTGTGAGGTGA TCGGCTTGCCATAGTAGTGG	96 652	20 20	60.107 59.536	50.000 55.000	557 bp
3	Forward primer Reverse primer	AGTTTGGCGTTGTGAGGTGA CGGCTTGCCATAGTAGTGGG	96 651	20 20	60.107 60.533	50.000 60.000	556 bp
4	Forward primer Reverse primer	TTGGCGTTGTGAGGTGAACT TCGGCTTGCCATAGTAGTGG	99 652	20 20	60.107 59.536	50.000 55.000	554 bp
5	Forward primer Reverse primer	TTGGCGTTGTGAGGTGAACT CGGCTTGCCATAGTAGTGGG	99 651	20 20	60.107 60.533	50.000 60.000	553 bp

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