HRGFish

A resource on hypoxia responsive genes in fish

Primer design

Genomic mRNA primers of the gene bnip3 (BCL2 interacting protein 3) of species Danio rerio

Selected sub sequence

Target sequence length: 14055

Selected subsequence from: 1 to:700

Selected subsequence size:700

GTAGAGTTGGCTCAATCTGGCAACCCTTGTGCCAGGGTGCCAGTTTTGTTGTCTTTCTGTTTGCCCTATATTTCCATCCATAGGACAGCCGCATGTTGCGTCACCACCAGCTGTGTGGAT
TCATGGAATAAAGATCCCGAACCAATTCTGTCACATGAATCCACATGCGGTTTATTGTGCTGGACGTGCAGAATATATCTAACAGAGCGCTGTCTCATCGACAAACACCTCGTTTTACTC
GTGGGGCGACCCATGTCTCTGGATAACACTGCGTTTCTGTATCTATCGACTGAATTTTTAAGGATTTAAACGGAGGTGAATCGTCCTTTTGTTGGTGAAAGAAACAAAAATGTCGATTGA
AAAGCACAGCGTGTCTGAAGAAAACCTTCAGGGTATGTAGATATCAAAACAACTCCGGTTAACGCTCGTTTTGTGCATGTTTCTCCTCTAAAACGCACATTGTTACCATGTAAATGCATG
TTTGGGGAATATTTACAGTTACATACGTATATTAAACGATGTTCTTTCACCTTAGTCTTTTATATTAATATTTGACAAGATGAAGCAGGTGTGTGTGTGTGTGTGTGTGTGTGTGTACAA
ACCACAAAAATGACGCATGTGATCCGCAATAATAAACACAGACGCGAAGCAAACTCTGAATCGTTAATGAATCATTCTTCGGAGTCGGATCTTTTAAACA

Primers information

S.No.	Primers	Sequences	Start	Length	Tm	GC %	Product size
1	Forward primer Reverse primer	ATCCATAGGACAGCCGCATG GTTTGCTTCGCGTCTGTGTT	75 653	20 20	59.964 59.972	55.000 50.000	579 bp
2	Forward primer Reverse primer	CATCCATAGGACAGCCGCAT TCAGAGTTTGCTTCGCGTCT	74 658	20 20	59.964 59.968	55.000 50.000	585 bp
3	Forward primer Reverse primer	ATCCATAGGACAGCCGCATG TCAGAGTTTGCTTCGCGTCT	75 658	20 20	59.964 59.968	55.000 50.000	584 bp
4	Forward primer Reverse primer	CATCCATAGGACAGCCGCAT GTTTGCTTCGCGTCTGTGTT	74 653	20 20	59.964 59.972	55.000 50.000	580 bp
5	Forward primer Reverse primer	ATCCATAGGACAGCCGCATG ACACACACACACCTGCTTCA	75 579	20 20	59.964 60.036	55.000 50.000	505 bp

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