HRGFish

A resource on hypoxia responsive genes in fish

Primer design

Genomic mRNA primers of the gene LOC103032190 (erythropoietin-like) of species Astyanax mexicanus

Selected sub sequence

Target sequence length: 21126

Selected subsequence from: 1 to:700

Selected subsequence size:700

AGCAGCGTGCAGCTAGGCAAAACAGTCGCATGGCTTTCAACAATGCACACCCTAAACATGCTAAACCTGGTTGCGAATGCCTTCAGTTTCTGGTAAGAATTGCTTTTTTCTCCTGCTGAT
GGGCTTACTTTGCATTTTTCTTTAAGGAATCTGGATTAAAAAAAATTATCATGCAAAAATAAAAAAACATAGAAATGTGTATTTAAAAGTAGGACAAGTGGGGTTCTGAAAAGGGTTTAA
TAAAAAAAATAAGCCTTGTTTTTTTGGTTTTACAGATATGCATATATACTCTGCATATAACTGGTCTTGTAAAACATGTGGCCATTATTAGGTGCACCTACTATTACCTTATTATCCCGC
TGAGTAAAGCGTGATACAGTAGACTGTAGGTCACCTGGTCACCAGTGTGTCAAGGACTGAAACTGTTCCCACGGAGAATGGCTAGCTCAAACTGTGCGTTTCCAATAAAATGTCCAGGAA
GTATTTCTTTAACTATGCAGGTCTTTATTTGACACACACGCTTAGCATCACAGCTTAATTACACCATGACCATAGAGCTACAGTTCTCTCCAAAAGCATTGGAGCAGTGAGGTCAATCCC
TTTTATTCTGCTGTAGACTGAGAACATTTGAAATTGACATTAAAGAATGACACAAAAGATGACACAAAAGATCCCCATTTTAGTTTTTATTTCCACGTAT

Primers information

S.No.	Primers	Sequences	Start	Length	Tm	GC %	Product size
1	Forward primer Reverse primer	CAGCTAGGCAAAACAGTCGC AGCTGTGATGCTAAGCGTGT	9 534	20 20	59.833 60.037	55.000 50.000	526 bp
2	Forward primer Reverse primer	CGTGCAGCTAGGCAAAACAG GACCTCACTGCTCCAATGCT	5 593	20 20	60.110 60.036	55.000 55.000	589 bp
3	Forward primer Reverse primer	CAGCTAGGCAAAACAGTCGC GACCTCACTGCTCCAATGCT	9 593	20 20	59.833 60.036	55.000 55.000	585 bp
4	Forward primer Reverse primer	GCAGCTAGGCAAAACAGTCG GACCTCACTGCTCCAATGCT	8 593	20 20	59.833 60.036	55.000 55.000	586 bp
5	Forward primer Reverse primer	CGTGCAGCTAGGCAAAACAG AGCTGTGATGCTAAGCGTGT	5 534	20 20	60.110 60.037	55.000 50.000	530 bp

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