HRGFish

A resource on hypoxia responsive genes in fish

Primer design

Genomic mRNA primers of the gene arnt2 (aryl hydrocarbon receptor nuclear translocator 2) of species Astyanax mexicanus

Selected sub sequence

Target sequence length: 155022

Selected subsequence from: 1 to:700

Selected subsequence size:700

GGGCGCGCGGTAATGTATGCAGGCTGAAGTCTCGCGGTGTTTCCGCACCTTTCAGACATCTTGCGTTATTTTTTCTGTAGCGAAGCTCCGCAGCAGCAGAGCCAGGGCTCCGTACAGCAG
GGCGGCACGGCCATCAACCCACACAGCCCGCGGTAAACACCCGCCCGCCCCTCCTACACCCTCCGCCCCGCGATCGGGCGGCTCGAGCTGCTGGCCCGGAGCTCGCCACTCGCTGCGTGC
GTCACCACCGTCCAGATCCCCGGGCTTACCGACGCCGCCGCCGCCGCTGCTGTTATTATTGTTGTGTCCGTTTTTCTCCGGCGAGCAGCGGAGCATGGCGACCCCCGCCGCGGTGAACCC
CTCCGGTAAGTCTGATCTTTGATTATCGAGGGTTTGATCGGGGGAGACTGGCTCCGGTGCTCCTGCGCGAGTGACGTTTTCCCGGTAACAAAAGAAAGGATCGGTGGGTGAAGAGGGAGC
ATCACCCCTCCTCCTCCTCCTCCTGTTTCTCCTCCTGCTCCTGATCCTTCATCACCTCCTCTTCCTCGCTGTGCCAGCCCTGCTTTAACATCCACACTAACACCAGCTGATCACTGCTCT
CACAGGCTCTGAGCTGATCTTTAATGGTTGGTAAGCTGATTATCCAGAGGAGAACGTTCCCTGTACTGGTAAGCTTGGCTAGATGGTTAACCAGGTCTGA

Primers information

S.No.	Primers	Sequences	Start	Length	Tm	GC %	Product size
1	Forward primer Reverse primer	TATGCAGGCTGAAGTCTCGC GGATCAGGAGCAGGAGGAGA	15 526	20 20	60.179 60.106	55.000 60.000	512 bp
2	Forward primer Reverse primer	TATGCAGGCTGAAGTCTCGC AGCGAGGAAGAGGAGGTGAT	15 549	20 20	60.179 60.033	55.000 55.000	535 bp
3	Forward primer Reverse primer	TATGCAGGCTGAAGTCTCGC GAGCAGTGATCAGCTGGTGT	15 598	20 20	60.179 60.037	55.000 55.000	584 bp
4	Forward primer Reverse primer	TATGCAGGCTGAAGTCTCGC AGGATCAGGAGCAGGAGGAG	15 527	20 20	60.179 60.106	55.000 60.000	513 bp
5	Forward primer Reverse primer	TATGCAGGCTGAAGTCTCGC GCGAGGAAGAGGAGGTGATG	15 548	20 20	60.179 59.896	55.000 60.000	534 bp

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