HRGFish

A resource on hypoxia responsive genes in fish

Primer design

Genomic mRNA primers of the gene arnt2 (aryl hydrocarbon receptor nuclear translocator 2) of species Takifugu rubripes

Selected sub sequence

Target sequence length: 38411

Selected subsequence from: 1 to:700

Selected subsequence size:700

ATAATAATTTATTCAAGTAACCCTAAATCTCGCGGTGCTACCATTCAGACATCTTGCATTTAATTAGAGCTTCACTTGGAGGGTTTTTCCTTTTCAACATACATGCACAGTTTCTCCATG
TTTTGAGGAAAAAAGCAACATTTATTCAAAGCCAGAATTTAGGTTACATGTCCTTTGTTTTAACGACAACGAGGCTGTTGTACCGACCGTCGCCTGCCAATCAACCCCCCGACATTAGAC
CGTATCTAGCGGGGCTGCTTCGTCGTATTGTGGACTATTTCCATCGCTGCGGCTTTATTTCCCTACGTTGAAAGGATGGCAACCCCCGCTGCCGTCAACCCATCGGGTAAGTAAATCCCT
CCTGTAACGTTACGAGGCAAAATGAGCGCTGAAAAAGACATAATGGTAAAGTAGCTGATATTTTTTTCGACGCTTGCCGAAGATGGCTGTCCCGACAGGCCGATGAAGCCTCGCCAGAGC
GTCCATCCCCCTTCCCCCTCCGACCTGCTCTTAACGCTGCCCTAGTAACGTTTTCGTGGCTGGCATCTTTTGCTGAATGCTATAAGGTCTTCGCTACATTGATATGACAGTGATCCACTT
TTACCTTGGCGATCTGCGTTATAATTACTCATATCAAAGCTATTTCGCATCTGCTCAATCATATACGAACATTACTTGCCAAATGTATTGGGGGTGTTGA

Primers information

S.No.	Primers	Sequences	Start	Length	Tm	GC %	Product size
1	Forward primer Reverse primer	ATCTCGCGGTGCTACCATTC ACGCAGATCGCCAAGGTAAA	26 618	20 20	60.249 60.037	55.000 50.000	593 bp
2	Forward primer Reverse primer	AATCTCGCGGTGCTACCATT ACGCAGATCGCCAAGGTAAA	25 618	20 20	59.822 60.037	50.000 50.000	594 bp
3	Forward primer Reverse primer	AAATCTCGCGGTGCTACCAT ACGCAGATCGCCAAGGTAAA	24 618	20 20	59.822 60.037	50.000 50.000	595 bp
4	Forward primer Reverse primer	AAATCTCGCGGTGCTACCAT AACGCAGATCGCCAAGGTAA	24 619	20 20	59.822 60.037	50.000 50.000	596 bp
5	Forward primer Reverse primer	AATCTCGCGGTGCTACCATT AACGCAGATCGCCAAGGTAA	25 619	20 20	59.822 60.037	50.000 50.000	595 bp

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